Complement system diagnostics

In the Department of Immunology, we determine individual factors of the complement system and carry out functional tests for all three activation pathways in order to map the function of the complement system.

Our service

  • Total complement activity (classic, alternative and MBL pathway)
  • Complement factor C3 and C4 for the question of excessive activation
  • C1 esterase inhibitor quantitative (serum) and functional (citrate) together with C4 determination if angioedema is suspected
  • Complement factor C1q to differentiate between hereditary and acquired angioedema (the C1q level is reduced in most cases of acquired, but not hereditary angioedema due to increased consumption; C1q deficiency is also associated with an increased risk of systemic lupus erythematosus )
  • Complement factor C2 in the clarification of frequent bacterial infections (C2 deficiency is also associated with an increased risk of systemic lupus erythematosus)
  • Complement factor H (regulatory factor) in the clarification of uncontrolled activation of the complement pathway in the context of atypical hemolytic uremic syndrome (aHUS) or dense deposition disease (membranoproliferative glomerulonephritis type II)
  • Complement factor B in clarifications regarding activation of the alternative complement pathway (rare cases of factor B deficiency are described, which lead to an increased incidence of bacterial infections)
  • Soluble complement complex (sC5b-9) in the clarification of complement defects, complement consumption in nephritis, vasculitis, meningitis, cryoglobulinemia, collagenoses, immune complex diseases, transfusion incidents, C3 glomerulopathy, atypical hemolytic uremic syndrome and in suspected immunodeficiency in recurrent infections as a follow-up.

The complement system is the backbone of the natural immune system and was discovered over 100 years ago on the assumption that it complemented the immune cells known at the time. Since then, over 50 components of this system have been described. Depending on the trigger, a distinction is made between three different activation pathways of the complement system: the classic, the alternative and the lectin pathway.

At the end of the three pathways, the membrane attack complex (C5b-9) is formed, which can kill pathogens and cells directly. Highly effective substances are produced as “by-products”. Some mark (opsonize) particles so that they can be phagocytosed by phagocytes, others are pro-inflammatory and attract immune cells.

In rare cases, there is a defective or reduced production of factors of the complement system, which can lead to a complement deficiency and thus to an increased susceptibility to infection or to increased autoimmunity. If the cause of the complement deficiency is congenital, the affected patients often already have severe infectious diseases as children, such as meningococcal meningitis or severe pneumonia. In some diseases, such as systemic lupus erythematosus or hereditary angioedema, complement factors are consumed faster than they are produced and can therefore be an indication or measure of disease activity.

Complement determinations are therefore indicated in the following cases: in the case of immunodeficiencies, e.g. if repeated infections with encapsulated pathogens have occurred, for the detection of increased activation or insufficient regulation in the context of autoimmune diseases and nephritides. Another indication is the determination of the C1 esterase inhibitor in angioedema.

Order card for referrers

You can use the order card to order individual analyses. To ensure that your order can be processed properly, it is essential that the order form is completed correctly.

 

We will gladly accept your order.

Complement system Order

If you send several orders at the same time, please enclose at least one sample tube with each order card.

Contact

If you have any questions, we will be happy to help you.

Tel. +41 44 255 12 00

You can reach us here:

Mon – Fri 8.00 – 12.00 and 13.00 – 17.00

Responsible Department